Correlation between microdilution, Etest, and disk diffusion methods for antifungal susceptibility testing of fluconazole against Candida sp. blood isolates

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Correlation between microdilution, Etest, and disk diffusion methods for antifungal susceptibility testing of fluconazole against Candida sp. blood isolates.

INTRODUCTION Antifungal susceptibility testing assists in finding the appropriate treatment for fungal infections, which are increasingly common. However, such testing is not very widespread. There are several existing methods, and the correlation between such methods was evaluated in this study. METHODS The susceptibility to fluconazole of 35 strains of Candida sp. isolated from blood cultur...

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Correlation between E-test, disk diffusion, and microdilution methods for antifungal susceptibility testing of fluconazole and voriconazole.

The activities of fluconazole and voriconazole against isolates of Candida spp. (n = 400) were tested by the E-test, disk diffusion, and the National Committee for Clinical Laboratory Standards (NCCLS) M27-A2 broth microdilution-based reference methods. More than 96% of isolates found to be susceptible to fluconazole by the reference method were identified as susceptible by the agar-based metho...

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Evaluation of Etest and disk diffusion methods compared with broth microdilution antifungal susceptibility testing of clinical isolates of Candida spp. against posaconazole.

We performed Etest, disk diffusion, and broth microdilution susceptibility testing of 2,171 clinical isolates of Candida spp. against posaconazole. By using provisional breakpoints for comparison purposes only, the categorical agreement between the agar-based methods and broth microdilution results ranged from 93 to 98%, with <1% very major errors. The essential agreement (within 2 well dilutio...

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Activities of amphotericin B, fluconazole and voriconazole against Candida bloodstream isolates determined by broth microdilution and disk diffusion methods

Objective: Invasive fungal infections caused by Candida species have increased significantly. Candidemia is not only associated with a mortality, but also extends the duration of hospital stay and increases the cost for medical care. Although, the clinical presentations of the patients with candidemia caused by Candida albicans and non-albicans Candida species are indistinguishable, the suscept...

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Filamentous Fungi Posaconazole against Clinical Isolates of Antifungal Susceptibility Testing of Compared to Broth Microdilution for Evaluation of Disk Diffusion and Etest

Published Ahead of Print 31 January 2007. 10.1128/JCM.02450-06. 2007, 45(4):1322. DOI: J. Clin. Microbiol. Michael A. Pfaller Linda B. Boyken, Shailesh Tendolkar, Jennifer Kroeger and Shawn A. Messer, Daniel J. Diekema, Richard J. Hollis, Filamentous Fungi Posaconazole against Clinical Isolates of Antifungal Susceptibility Testing of Compared to Broth Microdilution for Evaluation of Disk Diffus...

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ژورنال

عنوان ژورنال: Revista da Sociedade Brasileira de Medicina Tropical

سال: 2013

ISSN: 1678-9849,0037-8682

DOI: 10.1590/0037-868210502013